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Induced mutation and in vitro techniques as a method to induce salt tolerance in Basmati rice (Oryza sativa L.)
Article 7: Volume 2, Number 2, Summer 2005, Pages 141-146 (6) XML PDF (86 K)
Authors
M. Y. Saleem; Z. Mukhtar; A. A. Cheema; B. M. Atta
Abstract
Embryogenic callus of indica rice (Oryza sativa L.) cv. Basmati 370 induced on MS medium containing 9.05 ?M 2, 4-D was irradiated at 50 Gy of gamma rays of 60Co for creating genetic variability against salinity. Irradiated and non-irradiated calluses were screened in vitro through three consecutive proliferation phases at 4.0, 6.0, 8.0 and 10.0 d/Sm electrical conductivity of NaCl. Growth value and number of adapted mutagenized callus was more than that of non-mutagenized callus. Salinity levels beyond 6 d/Sm and 8 d/Sm were lethal to growth and adaptation of non-irradiated and irradiated callus respectively. NaCl adapted irradiated callus showed 2.0%-4.75% regeneration frequency on MS regeneration medium containing 5.37 M NAA and 9.29 ?M Kinetin. Non-mutagenized salt adapted callus did not show any regeneration. From gamma ray mutagenized cultures, 2 putative lines (M2 generation) with moderate salt tolerance were obtained at seedling stage. The results suggest that in vitro technique in connection with gamma rays may be used as a versatile approach to improve the level of salt tolerance in Basmati rice for saline environment.
Keywords
Callus; Induced mutation; NaCl stress; Regeneration; Rice
Main Subjects
Basmati rice; Oryza sativa L.; Mutation; In vitro; Salt tolerance
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